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primary human airway smooth muscle (asm) cells  (Lonza)


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    Structured Review

    Lonza primary human airway smooth muscle (asm) cells
    A) Relative GSDMB mRNA expression in human primary airway smooth muscle <t>(ASM),</t> <t>lung</t> <t>fibroblasts</t> (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.
    Primary Human Airway Smooth Muscle (Asm) Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+airway+smooth+muscle+(asm)+cells/airway+smooth+muscle+cells/pmc06037620-126-0-15
    Average 90 stars, based on 1 article reviews
    primary human airway smooth muscle (asm) cells - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "A functional splicing variant associated with decreased asthma risk abolishes the ability of gasdermin B ( GSMDB ) to induce epithelial cell pyroptosis"

    Article Title: A functional splicing variant associated with decreased asthma risk abolishes the ability of gasdermin B ( GSMDB ) to induce epithelial cell pyroptosis

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2017.11.040

    A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.
    Figure Legend Snippet: A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.

    Techniques Used: Expressing, Control, Immunostaining, Staining



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    Lonza primary human airway smooth muscle (asm) cells
    A) Relative GSDMB mRNA expression in human primary airway smooth muscle <t>(ASM),</t> <t>lung</t> <t>fibroblasts</t> (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.
    Primary Human Airway Smooth Muscle (Asm) Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+airway+smooth+muscle+(asm)+cells/airway+smooth+muscle+cells/pmc06037620-126-0-15
    Average 90 stars, based on 1 article reviews
    primary human airway smooth muscle (asm) cells - by Bioz Stars, 2026-08
    90/100 stars
      Buy from Supplier

    Image Search Results


    A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.

    Journal: The Journal of allergy and clinical immunology

    Article Title: A functional splicing variant associated with decreased asthma risk abolishes the ability of gasdermin B ( GSMDB ) to induce epithelial cell pyroptosis

    doi: 10.1016/j.jaci.2017.11.040

    Figure Lengend Snippet: A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.

    Article Snippet: Primary human airway smooth muscle (ASM) cells and normal human lung fibroblasts were obtained from Lonza.

    Techniques: Expressing, Control, Immunostaining, Staining