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primary human airway smooth muscle (asm) cells  (Lonza)


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    Lonza primary human airway smooth muscle (asm) cells
    A) Relative GSDMB mRNA expression in human primary airway smooth muscle <t>(ASM),</t> <t>lung</t> <t>fibroblasts</t> (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.
    Primary Human Airway Smooth Muscle (Asm) Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+airway+smooth+muscle+(asm)+cells/airway+smooth+muscle+cells/pmc06037620-126-0-15
    Average 90 stars, based on 1 article reviews
    primary human airway smooth muscle (asm) cells - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "A functional splicing variant associated with decreased asthma risk abolishes the ability of gasdermin B ( GSMDB ) to induce epithelial cell pyroptosis"

    Article Title: A functional splicing variant associated with decreased asthma risk abolishes the ability of gasdermin B ( GSMDB ) to induce epithelial cell pyroptosis

    Journal: The Journal of allergy and clinical immunology

    doi: 10.1016/j.jaci.2017.11.040

    A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.
    Figure Legend Snippet: A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.

    Techniques Used: Expressing, Control, Immunostaining, Staining

    Related Articles

    other:

    Article Title: A functional splicing variant associated with decreased asthma risk abolishes the ability of gasdermin B ( GSMDB ) to induce epithelial cell pyroptosis
    Article Snippet: Primary human airway smooth muscle (ASM) cells and normal human lung fibroblasts were obtained from Lonza.

    Disruption:

    Article Title: Heat Shock–Related Protein 20 Peptide Decreases Human Airway Constriction Downstream of β2-Adrenergic Receptor
    Article Snippet: .. Measurement of Stress fiber disruption and migration in human ASM cells Primary HASM cells (Lonza Group Ltd. (Basel, Germany) were grown in growth medium (Lonza) containing 5% fetal bovine serum, insulin, human epidermal growth factor, human fibroblast growth factor B and gentamicin and were maintained in a 37°C and 5% CO2 incubator. ..

    Article Title: Heat Shock–Related Protein 20 Peptide Decreases Human Airway Constriction Downstream of β2-Adrenergic Receptor
    Article Snippet: The ratio of filamentous (F)-actin versus globular (G)-actin in intact porcine ASM (PASM) tissue was measured using the G-actin/F-actin In Vivo Assay kit (Cytoskeleton, Denver, CO), per manufacturer’s protocol as described previously (30). .. Copyright © 2016 by the American Thoracic Society 10 Measurement of stress fiber disruption and migration in human ASM cells Primary HASM cells (Lonza Group Ltd.,Basel, Germany) were serum starved, either left untreated or treated with CCH (0.15 μM), 200 μM P20 peptides, or 200 μM P20 peptides followed by CCH (0.15 μM) and were stained with Alexa568-conjugated phalloidin and DAPI. ..

    Migration:

    Article Title: Heat Shock–Related Protein 20 Peptide Decreases Human Airway Constriction Downstream of β2-Adrenergic Receptor
    Article Snippet: .. Measurement of Stress fiber disruption and migration in human ASM cells Primary HASM cells (Lonza Group Ltd. (Basel, Germany) were grown in growth medium (Lonza) containing 5% fetal bovine serum, insulin, human epidermal growth factor, human fibroblast growth factor B and gentamicin and were maintained in a 37°C and 5% CO2 incubator. ..

    Article Title: Heat Shock–Related Protein 20 Peptide Decreases Human Airway Constriction Downstream of β2-Adrenergic Receptor
    Article Snippet: The ratio of filamentous (F)-actin versus globular (G)-actin in intact porcine ASM (PASM) tissue was measured using the G-actin/F-actin In Vivo Assay kit (Cytoskeleton, Denver, CO), per manufacturer’s protocol as described previously (30). .. Copyright © 2016 by the American Thoracic Society 10 Measurement of stress fiber disruption and migration in human ASM cells Primary HASM cells (Lonza Group Ltd.,Basel, Germany) were serum starved, either left untreated or treated with CCH (0.15 μM), 200 μM P20 peptides, or 200 μM P20 peptides followed by CCH (0.15 μM) and were stained with Alexa568-conjugated phalloidin and DAPI. ..

    Staining:

    Article Title: Heat Shock–Related Protein 20 Peptide Decreases Human Airway Constriction Downstream of β2-Adrenergic Receptor
    Article Snippet: The ratio of filamentous (F)-actin versus globular (G)-actin in intact porcine ASM (PASM) tissue was measured using the G-actin/F-actin In Vivo Assay kit (Cytoskeleton, Denver, CO), per manufacturer’s protocol as described previously (30). .. Copyright © 2016 by the American Thoracic Society 10 Measurement of stress fiber disruption and migration in human ASM cells Primary HASM cells (Lonza Group Ltd.,Basel, Germany) were serum starved, either left untreated or treated with CCH (0.15 μM), 200 μM P20 peptides, or 200 μM P20 peptides followed by CCH (0.15 μM) and were stained with Alexa568-conjugated phalloidin and DAPI. ..



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    Lonza primary human airway smooth muscle (asm) cells
    A) Relative GSDMB mRNA expression in human primary airway smooth muscle <t>(ASM),</t> <t>lung</t> <t>fibroblasts</t> (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.
    Primary Human Airway Smooth Muscle (Asm) Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+human+airway+smooth+muscle+(asm)+cells/airway+smooth+muscle+cells/pmc06037620-126-0-15
    Average 90 stars, based on 1 article reviews
    primary human airway smooth muscle (asm) cells - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.

    Journal: The Journal of allergy and clinical immunology

    Article Title: A functional splicing variant associated with decreased asthma risk abolishes the ability of gasdermin B ( GSMDB ) to induce epithelial cell pyroptosis

    doi: 10.1016/j.jaci.2017.11.040

    Figure Lengend Snippet: A) Relative GSDMB mRNA expression in human primary airway smooth muscle (ASM), lung fibroblasts (FIB), and normal human bronchial epithelial (NHBE) cells over the course of air-liquid interface (ALI) culture. ACTB (β-actin) was used as an internal control. B) Relative GSDMB mRNA expression in sorted β−tubulin IV-positive ciliated NHBE and MUC5AC-postive goblet NHBE cells. Graphs show mean of fold-change from three different donors; n=3, +/− SEM. C and D) Immunostaining of GSDMB protein in NHBE (normal human bronchial epithelial) cells at day 21 of ALI culture (C) and lung tissue (D) shows GSDMB expression in ciliated cells. Ciliated cells were stained for β−tubulin IV and nuclei were visualized by DAPI staining.

    Article Snippet: Primary human airway smooth muscle (ASM) cells and normal human lung fibroblasts were obtained from Lonza.

    Techniques: Expressing, Control, Immunostaining, Staining